klo-1
C. elegans
Glycoside hydrolase family 1 protein
What the model saw
Gene: klo-1 Organism: Caenorhabditis elegans Known functions: Glycoside hydrolase family 1 protein. beta-glucosidase activity. hydrolase activity, hydrolyzing O-glycosyl compounds.
View unredacted entry
Glycoside hydrolase family 1 protein. beta-glucosidase activity. hydrolase activity, hydrolyzing O-glycosyl compounds.
Solver output
klo-1 encodes a Glycoside hydrolase family 1 (GH1) beta-glucosidase that hydrolyzes O-glycosyl bonds, releasing glucose or other sugars from glycosylated substrates. While beta-glucosidases can influence nutrient availability and glycolipid/glycoprotein metabolism, the specific substrate(s) of klo-1 in C. elegans are not defined by these annotations alone. Beta-glucosidase activity could theoretically modulate ceramide levels (via glucosylceramide hydrolysis, relevant to lipid signaling and longevity) or affect carbohydrate metabolism, but without knowing the endogenous substrate, it is not possible to confidently assign a pro- or anti-longevity role. The GH1 family is broad and the molecular function annotations do not point to a specific aging-relevant pathway with sufficient clarity.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model predicted "unclear" with low confidence (0.35), while the ground truth is "pro_longevity." The model's reasoning is logically sound given the limited information provided — it correctly identifies that beta-glucosidase activity could theoretically influence longevity through multiple pathways but argues that without knowing the specific substrate, a confident assignment is not possible. This is a reasonable epistemic stance, but it is ultimately wrong. The model did not identify the correct longevity influence. The mechanistic reasoning is speculative but not incorrect per se — it just lacks the specific knowledge needed to connect klo-1 to pro-longevity effects. This is a case of appropriate uncertainty leading to a wrong answer rather than confident wrongness.
Raw API responses
solver
{
"reasoning": "klo-1 encodes a Glycoside hydrolase family 1 (GH1) beta-glucosidase that hydrolyzes O-glycosyl bonds, releasing glucose or other sugars from glycosylated substrates. While beta-glucosidases can influence nutrient availability and glycolipid/glycoprotein metabolism, the specific substrate(s) of klo-1 in C. elegans are not defined by these annotations alone. Beta-glucosidase activity could theoretically modulate ceramide levels (via glucosylceramide hydrolysis, relevant to lipid signaling and longevity) or affect carbohydrate metabolism, but without knowing the endogenous substrate, it is not possible to confidently assign a pro- or anti-longevity role. The GH1 family is broad and the molecular function annotations do not point to a specific aging-relevant pathway with sufficient clarity.",
"confidence": 0.35,
"key_pathways": [
"O-glycosyl hydrolysis",
"carbohydrate/lipid metabolism",
"glycolipid signaling"
],
"mechanism_class": "unclear",
"longevity_influence": "unclear"
}advisor
{
"notes": "The model predicted \"unclear\" with low confidence (0.35), while the ground truth is \"pro_longevity.\" The model's reasoning is logically sound given the limited information provided — it correctly identifies that beta-glucosidase activity could theoretically influence longevity through multiple pathways but argues that without knowing the specific substrate, a confident assignment is not possible. This is a reasonable epistemic stance, but it is ultimately wrong. The model did not identify the correct longevity influence. The mechanistic reasoning is speculative but not incorrect per se — it just lacks the specific knowledge needed to connect klo-1 to pro-longevity effects. This is a case of appropriate uncertainty leading to a wrong answer rather than confident wrongness.",
"failure_mode": "appropriately_uncertain",
"answer_correct": false,
"mechanism_correct": false,
"reasoning_quality": 3,
"ground_truth_questionable": false
}