OPT2
S. cerevisiae
Opt2p
What the model saw
Gene: OPT2 Organism: Saccharomyces cerevisiae Known functions: Opt2p. oligopeptide transmembrane transporter activity.
View unredacted entry
Opt2p. oligopeptide transmembrane transporter activity.
Solver output
OPT2 encodes a plasma membrane oligopeptide transporter (OPT family) in S. cerevisiae responsible for importing small peptides (typically 3–5 amino acids) into the cell. Peptide import can influence intracellular amino acid pools and thereby modulate TOR/TORC1 nutrient-sensing signaling, which is a central regulator of yeast longevity. However, the directionality of this effect is ambiguous: enhanced peptide uptake could either activate TOR (anti-longevity) by boosting amino acid availability, or support proteostasis and glutathione precursor supply (pro-longevity). OPT2 is a paralog of OPT1/HGT1, which transports glutathione, but OPT2's primary substrates and physiological role in aging are not well characterized. Without direct evidence linking OPT2 activity to a clear pro- or anti-longevity outcome, the prediction remains unclear.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model predicted "unclear" with low confidence (0.35), but the ground truth is "anti_longevity." The model's reasoning about OPT2's role in oligopeptide transport and potential TOR pathway modulation is plausible but ultimately inconclusive. The model correctly identified relevant pathways (nutrient sensing, amino acid pools) but failed to commit to the correct anti-longevity direction. The reasoning quality is modest - it acknowledges uncertainty appropriately but doesn't leverage enough specific knowledge about OPT2's deletion effects on yeast lifespan to make a confident prediction. The mechanism proposed (TOR/nutrient sensing) may or may not be correct for the actual anti-longevity effect.
Raw API responses
solver
{
"reasoning": "OPT2 encodes a plasma membrane oligopeptide transporter (OPT family) in S. cerevisiae responsible for importing small peptides (typically 3–5 amino acids) into the cell. Peptide import can influence intracellular amino acid pools and thereby modulate TOR/TORC1 nutrient-sensing signaling, which is a central regulator of yeast longevity. However, the directionality of this effect is ambiguous: enhanced peptide uptake could either activate TOR (anti-longevity) by boosting amino acid availability, or support proteostasis and glutathione precursor supply (pro-longevity). OPT2 is a paralog of OPT1/HGT1, which transports glutathione, but OPT2's primary substrates and physiological role in aging are not well characterized. Without direct evidence linking OPT2 activity to a clear pro- or anti-longevity outcome, the prediction remains unclear.",
"confidence": 0.35,
"key_pathways": [
"oligopeptide/amino acid import",
"TORC1 nutrient sensing",
"glutathione metabolism"
],
"mechanism_class": "deregulated_nutrient_sensing",
"longevity_influence": "unclear"
}advisor
{
"notes": "The model predicted \"unclear\" with low confidence (0.35), but the ground truth is \"anti_longevity.\" The model's reasoning about OPT2's role in oligopeptide transport and potential TOR pathway modulation is plausible but ultimately inconclusive. The model correctly identified relevant pathways (nutrient sensing, amino acid pools) but failed to commit to the correct anti-longevity direction. The reasoning quality is modest - it acknowledges uncertainty appropriately but doesn't leverage enough specific knowledge about OPT2's deletion effects on yeast lifespan to make a confident prediction. The mechanism proposed (TOR/nutrient sensing) may or may not be correct for the actual anti-longevity effect.",
"failure_mode": "appropriately_uncertain",
"answer_correct": false,
"mechanism_correct": false,
"reasoning_quality": 2,
"ground_truth_questionable": false
}