SCP160
S. cerevisiae
Scp160p
What the model saw
Gene: SCP160 Organism: Saccharomyces cerevisiae Known functions: Scp160p. G-protein alpha-subunit binding. RNA binding. mRNA binding. nucleic acid binding.
View unredacted entry
Scp160p. G-protein alpha-subunit binding. RNA binding. mRNA binding. nucleic acid binding.
Solver output
SCP160 encodes a polysome-associated RNA-binding protein (an hnRNP-like protein) in S. cerevisiae that binds mRNAs and interacts with G-protein alpha subunits, placing it at the intersection of mRNA surveillance/translational regulation and GPCR signaling. Its mRNA-binding activity links it to post-transcriptional gene regulation and translational control, which are critical for proteostasis and stress responses that influence replicative lifespan. Loss of SCP160 has been associated with defects in mRNA localization and translational fidelity, which can impair the cell's ability to respond to nutrient and stress signals. The G-protein alpha-subunit binding function connects SCP160 to pheromone/nutrient sensing pathways (Gpa1/cAMP-PKA axis), which are well-established modulators of yeast longevity. Proper regulation of these signaling cascades by SCP160 is expected to support, rather than undermine, longevity. Overall, the combination of mRNA stabilization/translational regulation and modulation of nutrient-sensing G-protein signaling suggests a net pro-longevity role.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model predicted pro_longevity while the ground truth is anti_longevity. The model's reasoning is plausible-sounding but ultimately incorrect - it assumed that SCP160's role in mRNA binding and G-protein signaling would support longevity, when in fact SCP160 deletion extends lifespan (making it anti-longevity). The model correctly identified the key functional domains (RNA binding, G-protein alpha subunit interaction) but drew the wrong conclusion about their net effect on longevity. The reasoning about "proper regulation supporting longevity" was speculative and led to the wrong answer. The confidence of 0.55 is relatively low, which is appropriate given the uncertainty, but the prediction was still wrong.
Raw API responses
solver
{
"reasoning": "SCP160 encodes a polysome-associated RNA-binding protein (an hnRNP-like protein) in S. cerevisiae that binds mRNAs and interacts with G-protein alpha subunits, placing it at the intersection of mRNA surveillance/translational regulation and GPCR signaling. Its mRNA-binding activity links it to post-transcriptional gene regulation and translational control, which are critical for proteostasis and stress responses that influence replicative lifespan. Loss of SCP160 has been associated with defects in mRNA localization and translational fidelity, which can impair the cell's ability to respond to nutrient and stress signals. The G-protein alpha-subunit binding function connects SCP160 to pheromone/nutrient sensing pathways (Gpa1/cAMP-PKA axis), which are well-established modulators of yeast longevity. Proper regulation of these signaling cascades by SCP160 is expected to support, rather than undermine, longevity. Overall, the combination of mRNA stabilization/translational regulation and modulation of nutrient-sensing G-protein signaling suggests a net pro-longevity role.",
"confidence": 0.55,
"key_pathways": [
"mRNA binding and translational regulation at polysomes",
"G-protein alpha-subunit (Gpa1) / cAMP-PKA nutrient sensing",
"post-transcriptional mRNA surveillance and localization"
],
"mechanism_class": "altered_intercellular_communication",
"longevity_influence": "pro_longevity"
}advisor
{
"notes": "The model predicted pro_longevity while the ground truth is anti_longevity. The model's reasoning is plausible-sounding but ultimately incorrect - it assumed that SCP160's role in mRNA binding and G-protein signaling would support longevity, when in fact SCP160 deletion extends lifespan (making it anti-longevity). The model correctly identified the key functional domains (RNA binding, G-protein alpha subunit interaction) but drew the wrong conclusion about their net effect on longevity. The reasoning about \"proper regulation supporting longevity\" was speculative and led to the wrong answer. The confidence of 0.55 is relatively low, which is appropriate given the uncertainty, but the prediction was still wrong.",
"failure_mode": "confident_wrong",
"answer_correct": false,
"mechanism_correct": false,
"reasoning_quality": 2,
"ground_truth_questionable": false
}