RPP2A
S. cerevisiae
ribosomal protein P2 RPP2A
What the model saw
Gene: RPP2A Organism: Saccharomyces cerevisiae Known functions: ribosomal protein P2 RPP2A. protein binding. protein kinase activator activity. structural constituent of ribosome.
View unredacted entry
ribosomal protein P2 RPP2A. protein binding. protein kinase activator activity. structural constituent of ribosome.
Solver output
RPP2A encodes a ribosomal stalk protein (P2) that is a structural constituent of the large ribosomal subunit and acts as a protein kinase activator. The ribosomal P-stalk complex (comprising P0, P1, P2) directly stimulates translational GTPases and promotes ribosome activity, thereby coupling nutrient availability to translational output — a core axis of TOR/nutrient-sensing signaling. Elevated ribosomal activity and enhanced translation are well-established pro-aging factors in yeast; deletion of ribosomal protein genes, including P-stalk components, frequently extends chronological and replicative lifespan in S. cerevisiae. The protein kinase activator activity of RPP2A may additionally amplify downstream signaling (e.g., via PKA or related kinases) that promotes growth at the expense of longevity. Reduced ribosome biogenesis and translation rate are hallmarks of dietary restriction-mediated lifespan extension, placing RPP2A's normal function in opposition to longevity.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model correctly predicted anti_longevity for RPP2A. The mechanistic reasoning is sound and well-grounded: ribosomal P-stalk proteins promote translation, and deletion of ribosomal protein genes in yeast frequently extends lifespan. The connection to TOR/nutrient-sensing signaling and dietary restriction is appropriate. The PKA connection is somewhat speculative but not incorrect. The confidence of 0.72 is reasonable. Overall a strong prediction with good mechanistic reasoning.
Raw API responses
solver
{
"reasoning": "RPP2A encodes a ribosomal stalk protein (P2) that is a structural constituent of the large ribosomal subunit and acts as a protein kinase activator. The ribosomal P-stalk complex (comprising P0, P1, P2) directly stimulates translational GTPases and promotes ribosome activity, thereby coupling nutrient availability to translational output — a core axis of TOR/nutrient-sensing signaling. Elevated ribosomal activity and enhanced translation are well-established pro-aging factors in yeast; deletion of ribosomal protein genes, including P-stalk components, frequently extends chronological and replicative lifespan in S. cerevisiae. The protein kinase activator activity of RPP2A may additionally amplify downstream signaling (e.g., via PKA or related kinases) that promotes growth at the expense of longevity. Reduced ribosome biogenesis and translation rate are hallmarks of dietary restriction-mediated lifespan extension, placing RPP2A's normal function in opposition to longevity.",
"confidence": 0.72,
"key_pathways": [
"TOR-mediated translational regulation",
"ribosomal P-stalk complex / translational GTPase activation",
"PKA/nutrient-sensing signaling"
],
"mechanism_class": "deregulated_nutrient_sensing",
"longevity_influence": "anti_longevity"
}advisor
{
"notes": "The model correctly predicted anti_longevity for RPP2A. The mechanistic reasoning is sound and well-grounded: ribosomal P-stalk proteins promote translation, and deletion of ribosomal protein genes in yeast frequently extends lifespan. The connection to TOR/nutrient-sensing signaling and dietary restriction is appropriate. The PKA connection is somewhat speculative but not incorrect. The confidence of 0.72 is reasonable. Overall a strong prediction with good mechanistic reasoning.",
"failure_mode": "correct",
"answer_correct": true,
"mechanism_correct": true,
"reasoning_quality": 4,
"ground_truth_questionable": false
}