ain-1
C. elegans
ALG-1 INteracting protein
What the model saw
Gene: ain-1 Organism: Caenorhabditis elegans Known functions: ALG-1 INteracting protein. protein binding.
View unredacted entry
ALG-1 INteracting protein. protein binding.
Solver output
AIN-1 is a GW182/TNRC6 ortholog in C. elegans that interacts with ALG-1, the Argonaute protein central to the miRNA-induced silencing complex (miRISC). AIN-1 functions as a scaffold within miRISC, bridging Argonaute-bound miRNAs to downstream effectors of translational repression and mRNA deadenylation. miRNA-mediated gene silencing via the ALG-1/AIN-1 complex is required for proper regulation of developmental timing and stress responses, including modulation of the DAF-2/insulin-IGF-1 signaling pathway through miRNAs such as miR-71 and let-7 family members. Loss of ain-1 disrupts miRISC function, impairing post-transcriptional silencing of pro-aging targets, and has been associated with reduced lifespan in C. elegans. Thus, the normal function of AIN-1 in supporting miRNA-mediated repression of aging-promoting transcripts is pro-longevity.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model correctly predicted pro_longevity for ain-1. The mechanistic reasoning is well-grounded: AIN-1 is indeed a GW182/TNRC6 ortholog that functions within the miRISC complex alongside ALG-1, and miRNA-mediated silencing is known to influence longevity in C. elegans. The connection to DAF-2/insulin-IGF-1 signaling via miRNAs like miR-71 and let-7 family members is plausible and consistent with the literature. The classification as "epigenetic_alterations" is a slight stretch — miRNA-mediated post-transcriptional silencing is more accurately described as post-transcriptional regulation rather than epigenetic alteration in the strict sense, but this is a minor categorization issue. Overall, the reasoning is solid and the answer is correct.
Raw API responses
solver
{
"reasoning": "AIN-1 is a GW182/TNRC6 ortholog in C. elegans that interacts with ALG-1, the Argonaute protein central to the miRNA-induced silencing complex (miRISC). AIN-1 functions as a scaffold within miRISC, bridging Argonaute-bound miRNAs to downstream effectors of translational repression and mRNA deadenylation. miRNA-mediated gene silencing via the ALG-1/AIN-1 complex is required for proper regulation of developmental timing and stress responses, including modulation of the DAF-2/insulin-IGF-1 signaling pathway through miRNAs such as miR-71 and let-7 family members. Loss of ain-1 disrupts miRISC function, impairing post-transcriptional silencing of pro-aging targets, and has been associated with reduced lifespan in C. elegans. Thus, the normal function of AIN-1 in supporting miRNA-mediated repression of aging-promoting transcripts is pro-longevity.",
"confidence": 0.62,
"key_pathways": [
"miRNA-induced silencing complex (miRISC)",
"ALG-1/Argonaute-mediated post-transcriptional regulation",
"DAF-2/insulin-IGF-1 signaling modulation via miRNAs"
],
"mechanism_class": "epigenetic_alterations",
"longevity_influence": "pro_longevity"
}advisor
{
"notes": "The model correctly predicted pro_longevity for ain-1. The mechanistic reasoning is well-grounded: AIN-1 is indeed a GW182/TNRC6 ortholog that functions within the miRISC complex alongside ALG-1, and miRNA-mediated silencing is known to influence longevity in C. elegans. The connection to DAF-2/insulin-IGF-1 signaling via miRNAs like miR-71 and let-7 family members is plausible and consistent with the literature. The classification as \"epigenetic_alterations\" is a slight stretch — miRNA-mediated post-transcriptional silencing is more accurately described as post-transcriptional regulation rather than epigenetic alteration in the strict sense, but this is a minor categorization issue. Overall, the reasoning is solid and the answer is correct.",
"failure_mode": "correct",
"answer_correct": true,
"mechanism_correct": true,
"reasoning_quality": 4,
"ground_truth_questionable": false
}