slcf-1
C. elegans
MFS domain-containing protein
What the model saw
Gene: slcf-1 Organism: Caenorhabditis elegans Known functions: MFS domain-containing protein. monocarboxylic acid transmembrane transporter activity. transmembrane transporter activity.
View unredacted entry
MFS domain-containing protein. monocarboxylic acid transmembrane transporter activity. transmembrane transporter activity.
Solver output
slcf-1 encodes a Major Facilitator Superfamily (MFS) domain-containing monocarboxylic acid transporter in C. elegans. MFS transporters in this class mediate the uptake or efflux of short-chain monocarboxylates such as lactate, pyruvate, acetate, and ketone bodies, which are key metabolic intermediates linking glycolysis, the TCA cycle, and mitochondrial energy metabolism. Proper transport of these metabolites is critical for maintaining metabolic homeostasis and nutrient sensing, particularly in the context of dietary restriction and lipid/carbohydrate partitioning. In C. elegans, disruption of monocarboxylate transport can impair mitochondrial substrate supply and alter the NAD+/NADH ratio, which feeds into sirtuins and AMPK signaling — pathways central to longevity regulation. The net effect of normal transporter function is to support efficient energy metabolism and metabolic flexibility, which is generally associated with healthy aging and lifespan extension. However, the directionality is uncertain because some MFS transporters can also facilitate nutrient uptake that activates pro-aging mTOR/insulin signaling.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model predicted pro_longevity but the ground truth is anti_longevity. The model's reasoning is generic speculation about MFS transporters and monocarboxylate metabolism without any specific knowledge of slcf-1's actual role in C. elegans longevity. The model even acknowledges uncertainty about directionality but still chose the wrong direction. The mechanistic reasoning is not grounded in actual experimental data about slcf-1 - it's generic pathway reasoning that could apply to any monocarboxylate transporter. The confidence of 0.52 is low but the model still committed to the wrong answer. The actual anti-longevity role of slcf-1 (loss of function extends lifespan) is not captured in the reasoning.
Raw API responses
solver
{
"reasoning": "slcf-1 encodes a Major Facilitator Superfamily (MFS) domain-containing monocarboxylic acid transporter in C. elegans. MFS transporters in this class mediate the uptake or efflux of short-chain monocarboxylates such as lactate, pyruvate, acetate, and ketone bodies, which are key metabolic intermediates linking glycolysis, the TCA cycle, and mitochondrial energy metabolism. Proper transport of these metabolites is critical for maintaining metabolic homeostasis and nutrient sensing, particularly in the context of dietary restriction and lipid/carbohydrate partitioning. In C. elegans, disruption of monocarboxylate transport can impair mitochondrial substrate supply and alter the NAD+/NADH ratio, which feeds into sirtuins and AMPK signaling — pathways central to longevity regulation. The net effect of normal transporter function is to support efficient energy metabolism and metabolic flexibility, which is generally associated with healthy aging and lifespan extension. However, the directionality is uncertain because some MFS transporters can also facilitate nutrient uptake that activates pro-aging mTOR/insulin signaling.",
"confidence": 0.52,
"key_pathways": [
"monocarboxylate transport / metabolic substrate supply",
"deregulated nutrient sensing (AMPK/sirtuin axis)",
"mitochondrial energy metabolism"
],
"mechanism_class": "deregulated_nutrient_sensing",
"longevity_influence": "pro_longevity"
}advisor
{
"notes": "The model predicted pro_longevity but the ground truth is anti_longevity. The model's reasoning is generic speculation about MFS transporters and monocarboxylate metabolism without any specific knowledge of slcf-1's actual role in C. elegans longevity. The model even acknowledges uncertainty about directionality but still chose the wrong direction. The mechanistic reasoning is not grounded in actual experimental data about slcf-1 - it's generic pathway reasoning that could apply to any monocarboxylate transporter. The confidence of 0.52 is low but the model still committed to the wrong answer. The actual anti-longevity role of slcf-1 (loss of function extends lifespan) is not captured in the reasoning.",
"failure_mode": "confident_wrong",
"answer_correct": false,
"mechanism_correct": false,
"reasoning_quality": 2,
"ground_truth_questionable": false
}