ent-5
C. elegans
Equilibrative nucleoside transporter 1
What the model saw
Gene: ent-5 Organism: Caenorhabditis elegans Known functions: Equilibrative nucleoside transporter 1. nucleoside transmembrane transporter activity. transmembrane transporter activity.
View unredacted entry
Equilibrative nucleoside transporter 1. nucleoside transmembrane transporter activity. transmembrane transporter activity.
Solver output
ENT-5 is an equilibrative nucleoside transporter (ENT family) responsible for facilitating the bidirectional transport of nucleosides across membranes. Nucleoside transport is critical for nucleotide salvage pathways, which supply precursors for DNA repair, RNA synthesis, and NAD+ biosynthesis (e.g., transport of nicotinamide riboside). In C. elegans, ENT family members have been linked to NAD+ precursor uptake; adequate intracellular NAD+ levels support sirtuin activity and mitochondrial function, both of which are pro-longevity. Loss of nucleoside transporter function can impair NAD+ salvage, reduce sirtuin-mediated deacetylation, and compromise metabolic homeostasis, placing this gene in the nutrient-sensing axis. The equilibrative nature of the transporter also suggests a role in purine/pyrimidine homeostasis that supports genomic integrity and cellular energy balance.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model predicted pro_longevity but the ground truth is anti_longevity. The model's reasoning is speculative and built around NAD+/sirtuin pathways without direct evidence for ent-5 specifically. The model assumed that loss of ent-5 would impair NAD+ salvage and thus reduce lifespan (making it pro_longevity when present), but the actual classification is anti_longevity, meaning ent-5 activity works against longevity (i.e., its loss or reduction extends lifespan). The mechanistic reasoning is not supported by the actual biology of this gene. Confidence was moderate (0.55) but still wrong direction. The reasoning quality is poor because it relies on generic nucleoside transporter biology rather than any specific evidence about ent-5 in C. elegans aging.
Raw API responses
solver
{
"reasoning": "ENT-5 is an equilibrative nucleoside transporter (ENT family) responsible for facilitating the bidirectional transport of nucleosides across membranes. Nucleoside transport is critical for nucleotide salvage pathways, which supply precursors for DNA repair, RNA synthesis, and NAD+ biosynthesis (e.g., transport of nicotinamide riboside). In C. elegans, ENT family members have been linked to NAD+ precursor uptake; adequate intracellular NAD+ levels support sirtuin activity and mitochondrial function, both of which are pro-longevity. Loss of nucleoside transporter function can impair NAD+ salvage, reduce sirtuin-mediated deacetylation, and compromise metabolic homeostasis, placing this gene in the nutrient-sensing axis. The equilibrative nature of the transporter also suggests a role in purine/pyrimidine homeostasis that supports genomic integrity and cellular energy balance.",
"confidence": 0.55,
"key_pathways": [
"NAD+ salvage pathway",
"nucleoside/nucleotide homeostasis",
"sirtuin-mediated metabolic regulation"
],
"mechanism_class": "deregulated_nutrient_sensing",
"longevity_influence": "pro_longevity"
}advisor
{
"notes": "The model predicted pro_longevity but the ground truth is anti_longevity. The model's reasoning is speculative and built around NAD+/sirtuin pathways without direct evidence for ent-5 specifically. The model assumed that loss of ent-5 would impair NAD+ salvage and thus reduce lifespan (making it pro_longevity when present), but the actual classification is anti_longevity, meaning ent-5 activity works against longevity (i.e., its loss or reduction extends lifespan). The mechanistic reasoning is not supported by the actual biology of this gene. Confidence was moderate (0.55) but still wrong direction. The reasoning quality is poor because it relies on generic nucleoside transporter biology rather than any specific evidence about ent-5 in C. elegans aging.",
"failure_mode": "confident_wrong",
"answer_correct": false,
"mechanism_correct": false,
"reasoning_quality": 2,
"ground_truth_questionable": false
}