exc-14
C. elegans
RING-type domain-containing protein
What the model saw
Gene: exc-14 Organism: Caenorhabditis elegans Known functions: RING-type domain-containing protein. ubiquitin protein ligase activity.
View unredacted entry
RING-type domain-containing protein. ubiquitin protein ligase activity.
Solver output
exc-14 encodes a RING-type E3 ubiquitin ligase in C. elegans. E3 ubiquitin ligases direct substrate proteins for proteasomal degradation, which is a central mechanism in proteostasis maintenance. However, the longevity influence depends critically on which substrates are targeted: if exc-14 ubiquitinates pro-longevity factors (e.g., DAF-16/FOXO, HSF-1), it would be anti-longevity; if it targets pro-aging or growth-promoting proteins, it would be pro-longevity. The specific substrates and pathway context of exc-14 are not well characterized in the aging literature, and RING-domain E3 ligases as a class have highly variable effects on lifespan depending on their targets. Without substrate identity or pathway placement, the directionality of its effect on longevity cannot be confidently determined from molecular function annotations alone.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model predicted "unclear" while the ground truth is "pro_longevity." The model's reasoning is logically sound given the limited information provided - it correctly identifies exc-14 as a RING-type E3 ubiquitin ligase and notes that the longevity effect depends on substrate identity. However, it failed to commit to the correct answer. The model's uncertainty is understandable given the sparse annotation, but it still got the answer wrong. The mechanism class of "loss_of_proteostasis" is somewhat misapplied here - the gene is involved in proteostasis regulation, but framing it as "loss_of_proteostasis" is not quite right for an E3 ligase. The reasoning quality is decent in terms of logical structure but ultimately unhelpful for arriving at the correct answer.
Raw API responses
solver
{
"reasoning": "exc-14 encodes a RING-type E3 ubiquitin ligase in C. elegans. E3 ubiquitin ligases direct substrate proteins for proteasomal degradation, which is a central mechanism in proteostasis maintenance. However, the longevity influence depends critically on which substrates are targeted: if exc-14 ubiquitinates pro-longevity factors (e.g., DAF-16/FOXO, HSF-1), it would be anti-longevity; if it targets pro-aging or growth-promoting proteins, it would be pro-longevity. The specific substrates and pathway context of exc-14 are not well characterized in the aging literature, and RING-domain E3 ligases as a class have highly variable effects on lifespan depending on their targets. Without substrate identity or pathway placement, the directionality of its effect on longevity cannot be confidently determined from molecular function annotations alone.",
"confidence": 0.35,
"key_pathways": [
"ubiquitin-proteasome system",
"proteostasis regulation",
"RING-type E3 ubiquitin ligase activity"
],
"mechanism_class": "loss_of_proteostasis",
"longevity_influence": "unclear"
}advisor
{
"notes": "The model predicted \"unclear\" while the ground truth is \"pro_longevity.\" The model's reasoning is logically sound given the limited information provided - it correctly identifies exc-14 as a RING-type E3 ubiquitin ligase and notes that the longevity effect depends on substrate identity. However, it failed to commit to the correct answer. The model's uncertainty is understandable given the sparse annotation, but it still got the answer wrong. The mechanism class of \"loss_of_proteostasis\" is somewhat misapplied here - the gene is involved in proteostasis regulation, but framing it as \"loss_of_proteostasis\" is not quite right for an E3 ligase. The reasoning quality is decent in terms of logical structure but ultimately unhelpful for arriving at the correct answer.",
"failure_mode": "appropriately_uncertain",
"answer_correct": false,
"mechanism_correct": false,
"reasoning_quality": 3,
"ground_truth_questionable": false
}