SRS2
S. cerevisiae
DNA helicase SRS2
What the model saw
Gene: SRS2 Organism: Saccharomyces cerevisiae Known functions: DNA helicase SRS2. 3'-5' DNA helicase activity. ATP binding. ATP hydrolysis activity. DNA binding. DNA helicase activity. enzyme activator activity. hydrolase activity. protein binding.
View unredacted entry
DNA helicase SRS2. 3'-5' DNA helicase activity. ATP binding. ATP hydrolysis activity. DNA binding. DNA helicase activity. enzyme activator activity. hydrolase activity. protein binding.
Solver output
SRS2 encodes a 3'-5' DNA helicase in S. cerevisiae that functions as an anti-recombinase by dismantling Rad51 nucleoprotein filaments at stalled replication forks, thereby suppressing inappropriate or toxic homologous recombination intermediates. By channeling DNA damage repair away from error-prone recombination and toward template-switching or translesion synthesis pathways, SRS2 reduces the accumulation of recombination-associated genome rearrangements. Its ATP-dependent helicase and hydrolase activities are essential for this anti-recombination function. Loss of SRS2 leads to hyperrecombination, increased gross chromosomal rearrangements, and elevated mutation rates — all hallmarks of genomic instability that shorten replicative lifespan in yeast. Consistent with this, srs2 mutants show reduced replicative lifespan, supporting SRS2's normal function as pro-longevity through maintenance of genome integrity.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model correctly predicted SRS2 as pro_longevity with high confidence (0.72). The mechanistic reasoning is detailed and accurate: SRS2 acts as an anti-recombinase by dismantling Rad51 nucleoprotein filaments, suppressing toxic recombination intermediates, and maintaining genome integrity. The model correctly notes that srs2 mutants show reduced replicative lifespan in yeast, supporting the pro-longevity classification. The key pathways identified (HR suppression, DNA damage tolerance, replication fork stability) are all relevant and correct. This is a well-reasoned, accurate prediction.
Raw API responses
solver
{
"reasoning": "SRS2 encodes a 3'-5' DNA helicase in S. cerevisiae that functions as an anti-recombinase by dismantling Rad51 nucleoprotein filaments at stalled replication forks, thereby suppressing inappropriate or toxic homologous recombination intermediates. By channeling DNA damage repair away from error-prone recombination and toward template-switching or translesion synthesis pathways, SRS2 reduces the accumulation of recombination-associated genome rearrangements. Its ATP-dependent helicase and hydrolase activities are essential for this anti-recombination function. Loss of SRS2 leads to hyperrecombination, increased gross chromosomal rearrangements, and elevated mutation rates — all hallmarks of genomic instability that shorten replicative lifespan in yeast. Consistent with this, srs2 mutants show reduced replicative lifespan, supporting SRS2's normal function as pro-longevity through maintenance of genome integrity.",
"confidence": 0.72,
"key_pathways": [
"homologous recombination suppression (Rad51 filament disassembly)",
"DNA damage tolerance / template switching",
"replication fork stability"
],
"mechanism_class": "genomic_instability",
"longevity_influence": "pro_longevity"
}advisor
{
"notes": "The model correctly predicted SRS2 as pro_longevity with high confidence (0.72). The mechanistic reasoning is detailed and accurate: SRS2 acts as an anti-recombinase by dismantling Rad51 nucleoprotein filaments, suppressing toxic recombination intermediates, and maintaining genome integrity. The model correctly notes that srs2 mutants show reduced replicative lifespan in yeast, supporting the pro-longevity classification. The key pathways identified (HR suppression, DNA damage tolerance, replication fork stability) are all relevant and correct. This is a well-reasoned, accurate prediction.",
"failure_mode": "correct",
"answer_correct": true,
"mechanism_correct": true,
"reasoning_quality": 5,
"ground_truth_questionable": false
}