f42g10.1
C. elegans
Peptidase_M13 domain-containing protein
What the model saw
Gene: F42G10.1 Organism: Caenorhabditis elegans Known functions: Peptidase_M13 domain-containing protein. metalloendopeptidase activity. metallopeptidase activity.
View unredacted entry
Peptidase_M13 domain-containing protein. metalloendopeptidase activity. metallopeptidase activity.
Solver output
F42G10.1 encodes a Peptidase_M13 domain-containing metalloendopeptidase in C. elegans. The M13 family (neprilysin-like peptidases) are zinc-dependent endopeptidases that cleave and inactivate extracellular signaling peptides, including neuropeptides and peptide hormones. In C. elegans, neprilysin-family enzymes (e.g., NEP-1, NEP-2) regulate neuropeptide signaling and have been linked to modulation of insulin/IGF-1-like signaling through peptide ligand degradation. Loss of neprilysin activity can alter intercellular peptide communication and affect DAF-16/FOXO-dependent longevity pathways. However, the directionality is ambiguous: degrading pro-aging peptide signals could be pro-longevity, while degrading pro-longevity peptides could be anti-longevity. The balance of evidence for M13 metallopeptidases in nematodes slightly favors a role in clearing peptides that would otherwise suppress longevity pathways, suggesting a net pro-longevity function, but confidence is low given the functional ambiguity.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model predicted pro_longevity but the ground truth is anti_longevity. The model correctly identified the M13 metallopeptidase family and acknowledged the ambiguity in directionality, but ultimately chose the wrong direction. The reasoning about neprilysin-like enzymes clearing peptides that suppress longevity pathways was speculative and incorrect. The model's confidence was relatively low (0.42), which is appropriate given the uncertainty, but it still landed on the wrong answer. The mechanistic reasoning was partially relevant (M13 family, neuropeptide signaling) but the conclusion about directionality was wrong.
Raw API responses
solver
{
"reasoning": "F42G10.1 encodes a Peptidase_M13 domain-containing metalloendopeptidase in C. elegans. The M13 family (neprilysin-like peptidases) are zinc-dependent endopeptidases that cleave and inactivate extracellular signaling peptides, including neuropeptides and peptide hormones. In C. elegans, neprilysin-family enzymes (e.g., NEP-1, NEP-2) regulate neuropeptide signaling and have been linked to modulation of insulin/IGF-1-like signaling through peptide ligand degradation. Loss of neprilysin activity can alter intercellular peptide communication and affect DAF-16/FOXO-dependent longevity pathways. However, the directionality is ambiguous: degrading pro-aging peptide signals could be pro-longevity, while degrading pro-longevity peptides could be anti-longevity. The balance of evidence for M13 metallopeptidases in nematodes slightly favors a role in clearing peptides that would otherwise suppress longevity pathways, suggesting a net pro-longevity function, but confidence is low given the functional ambiguity.",
"confidence": 0.42,
"key_pathways": [
"insulin/IGF-1 signaling (IIS) / DAF-16 pathway",
"neuropeptide signaling and degradation",
"extracellular peptide homeostasis"
],
"mechanism_class": "altered_intercellular_communication",
"longevity_influence": "pro_longevity"
}advisor
{
"notes": "The model predicted pro_longevity but the ground truth is anti_longevity. The model correctly identified the M13 metallopeptidase family and acknowledged the ambiguity in directionality, but ultimately chose the wrong direction. The reasoning about neprilysin-like enzymes clearing peptides that suppress longevity pathways was speculative and incorrect. The model's confidence was relatively low (0.42), which is appropriate given the uncertainty, but it still landed on the wrong answer. The mechanistic reasoning was partially relevant (M13 family, neuropeptide signaling) but the conclusion about directionality was wrong.",
"failure_mode": "confident_wrong",
"answer_correct": false,
"mechanism_correct": false,
"reasoning_quality": 2,
"ground_truth_questionable": false
}