CAN1
S. cerevisiae
arginine permease CAN1
What the model saw
Gene: CAN1 Organism: Saccharomyces cerevisiae Known functions: arginine permease CAN1. L-arginine transmembrane transporter activity. amino acid transmembrane transporter activity. basic amino acid transmembrane transporter activity. transmembrane transporter activity.
View unredacted entry
arginine permease CAN1. L-arginine transmembrane transporter activity. amino acid transmembrane transporter activity. basic amino acid transmembrane transporter activity. transmembrane transporter activity.
Solver output
CAN1 encodes the primary high-affinity arginine permease in S. cerevisiae, responsible for importing arginine and other basic amino acids into the cell. Elevated intracellular amino acid levels, particularly arginine, activate TORC1 (Target of Rapamycin Complex 1) signaling, a central nutrient-sensing hub whose hyperactivation is well-established to shorten lifespan in yeast and other organisms. CAN1 also functions as a sensor/transceptor at the plasma membrane that can directly stimulate PKA and TORC1 pathways upon amino acid binding, independent of transport. Furthermore, CAN1 is a known component of the eisosome/MCC (Membrane Compartment of Can1) and its activity promotes anabolic signaling that opposes the pro-longevity effects of nutrient restriction. Loss of CAN1 reduces arginine uptake, dampens TORC1 activity, and has been associated with extended chronological lifespan in yeast, consistent with an anti-longevity role for the normal (active) gene.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model correctly predicted anti_longevity for CAN1. The mechanistic reasoning is sound and well-articulated: CAN1 as an arginine permease promotes amino acid uptake, which activates TORC1 and PKA signaling, shortening lifespan. Loss of CAN1 reduces these pro-aging signals and extends chronological lifespan. The transceptor/eisosome details add plausibility. The reasoning is largely correct, though some specifics (e.g., direct PKA activation via transceptor function) may be somewhat speculative or overstated. Overall, a strong and well-reasoned prediction.
Raw API responses
solver
{
"reasoning": "CAN1 encodes the primary high-affinity arginine permease in S. cerevisiae, responsible for importing arginine and other basic amino acids into the cell. Elevated intracellular amino acid levels, particularly arginine, activate TORC1 (Target of Rapamycin Complex 1) signaling, a central nutrient-sensing hub whose hyperactivation is well-established to shorten lifespan in yeast and other organisms. CAN1 also functions as a sensor/transceptor at the plasma membrane that can directly stimulate PKA and TORC1 pathways upon amino acid binding, independent of transport. Furthermore, CAN1 is a known component of the eisosome/MCC (Membrane Compartment of Can1) and its activity promotes anabolic signaling that opposes the pro-longevity effects of nutrient restriction. Loss of CAN1 reduces arginine uptake, dampens TORC1 activity, and has been associated with extended chronological lifespan in yeast, consistent with an anti-longevity role for the normal (active) gene.",
"confidence": 0.72,
"key_pathways": [
"TORC1 nutrient sensing / amino acid signaling",
"Arginine/basic amino acid import and intracellular availability",
"PKA signaling activation via transceptor function"
],
"mechanism_class": "deregulated_nutrient_sensing",
"longevity_influence": "anti_longevity"
}advisor
{
"notes": "The model correctly predicted anti_longevity for CAN1. The mechanistic reasoning is sound and well-articulated: CAN1 as an arginine permease promotes amino acid uptake, which activates TORC1 and PKA signaling, shortening lifespan. Loss of CAN1 reduces these pro-aging signals and extends chronological lifespan. The transceptor/eisosome details add plausibility. The reasoning is largely correct, though some specifics (e.g., direct PKA activation via transceptor function) may be somewhat speculative or overstated. Overall, a strong and well-reasoned prediction.",
"failure_mode": "correct",
"answer_correct": true,
"mechanism_correct": true,
"reasoning_quality": 4,
"ground_truth_questionable": false
}