PHO84
S. cerevisiae
phosphate transporter PHO84
What the model saw
Gene: PHO84 Organism: Saccharomyces cerevisiae Known functions: phosphate transporter PHO84. manganese ion transmembrane transporter activity. phosphate transmembrane transporter activity. selenite:proton symporter activity. transmembrane transporter activity. zinc ion binding.
View unredacted entry
phosphate transporter PHO84. manganese ion transmembrane transporter activity. phosphate transmembrane transporter activity. selenite:proton symporter activity. transmembrane transporter activity. zinc ion binding.
Solver output
PHO84 is the high-affinity inorganic phosphate transporter in S. cerevisiae, responsible for phosphate uptake from the environment. Elevated intracellular phosphate imported by PHO84 activates the PKA/TOR nutrient-sensing pathway and suppresses stress-response programs, thereby shortening chronological lifespan. Deletion of PHO84 has been shown to extend chronological lifespan in yeast by reducing phosphate-driven activation of pro-aging nutrient-sensing cascades (TOR and PKA signaling), mimicking a nutrient-limitation state. PHO84's role as a proton-coupled symporter also links phosphate import to cytosolic acidification, which further modulates aging-related signaling. Thus, the normal function of PHO84 — actively importing phosphate — promotes nutrient-sensing pathway activity and opposes longevity.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model predicted PHO84 as anti_longevity with high confidence (0.82), but the ground truth is pro_longevity. The model's reasoning is internally consistent but factually incorrect — it claims that deletion of PHO84 extends chronological lifespan, which would make PHO84 anti-longevity. However, the GenAge database classifies PHO84 as pro_longevity, meaning its presence/activity promotes longevity rather than opposing it. The model's mechanistic narrative about PHO84 driving TOR/PKA activation and shortening lifespan is inverted from the actual biology as recorded in GenAge. This is a confident wrong answer with incorrect mechanistic reasoning.
Raw API responses
solver
{
"reasoning": "PHO84 is the high-affinity inorganic phosphate transporter in S. cerevisiae, responsible for phosphate uptake from the environment. Elevated intracellular phosphate imported by PHO84 activates the PKA/TOR nutrient-sensing pathway and suppresses stress-response programs, thereby shortening chronological lifespan. Deletion of PHO84 has been shown to extend chronological lifespan in yeast by reducing phosphate-driven activation of pro-aging nutrient-sensing cascades (TOR and PKA signaling), mimicking a nutrient-limitation state. PHO84's role as a proton-coupled symporter also links phosphate import to cytosolic acidification, which further modulates aging-related signaling. Thus, the normal function of PHO84 — actively importing phosphate — promotes nutrient-sensing pathway activity and opposes longevity.",
"confidence": 0.82,
"key_pathways": [
"TOR/PKA nutrient-sensing signaling",
"phosphate homeostasis (PHO pathway)",
"chronological lifespan regulation"
],
"mechanism_class": "deregulated_nutrient_sensing",
"longevity_influence": "anti_longevity"
}advisor
{
"notes": "The model predicted PHO84 as anti_longevity with high confidence (0.82), but the ground truth is pro_longevity. The model's reasoning is internally consistent but factually incorrect — it claims that deletion of PHO84 extends chronological lifespan, which would make PHO84 anti-longevity. However, the GenAge database classifies PHO84 as pro_longevity, meaning its presence/activity promotes longevity rather than opposing it. The model's mechanistic narrative about PHO84 driving TOR/PKA activation and shortening lifespan is inverted from the actual biology as recorded in GenAge. This is a confident wrong answer with incorrect mechanistic reasoning.",
"failure_mode": "confident_wrong",
"answer_correct": false,
"mechanism_correct": false,
"reasoning_quality": 2,
"ground_truth_questionable": true
}