APC5
S. cerevisiae
anaphase promoting complex subunit 5
What the model saw
Gene: APC5 Organism: Saccharomyces cerevisiae Known functions: anaphase promoting complex subunit 5. protein binding. ubiquitin protein ligase activity.
View unredacted entry
anaphase promoting complex subunit 5. protein binding. ubiquitin protein ligase activity.
Solver output
APC5 is a subunit of the Anaphase Promoting Complex/Cyclosome (APC/C), an E3 ubiquitin ligase that targets key cell cycle regulators—including cyclins (Clb2, Clb5) and securin (Pds1)—for proteasomal degradation. Proper APC/C activity is essential for orderly mitotic exit and prevention of aberrant cell cycle re-entry, which is a hallmark of cellular senescence. By ensuring timely ubiquitin-mediated degradation of mitotic cyclins, APC5 supports genomic stability and prevents the accumulation of cells arrested in aberrant cell cycle states. In yeast, defects in APC/C function lead to chromosome missegregation and genomic instability, both of which shorten replicative lifespan. Additionally, APC/C-mediated proteostasis of cell cycle regulators intersects with nutrient-sensing pathways that modulate chronological and replicative aging in S. cerevisiae.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The ground truth is "unclear" but the model predicted "pro_longevity" with 0.65 confidence. The model constructed a plausible-sounding mechanistic argument about APC/C function in cell cycle regulation and genomic stability, but this reasoning is speculative and not supported by direct experimental evidence in yeast aging studies. The model's confidence (0.65) is too high given the actual ambiguity of APC5's role in longevity. The mechanistic reasoning, while biologically coherent in general terms, is not specifically validated for APC5's role in yeast aging, making it hallucinated specifics applied to an unclear case.
Raw API responses
solver
{
"reasoning": "APC5 is a subunit of the Anaphase Promoting Complex/Cyclosome (APC/C), an E3 ubiquitin ligase that targets key cell cycle regulators—including cyclins (Clb2, Clb5) and securin (Pds1)—for proteasomal degradation. Proper APC/C activity is essential for orderly mitotic exit and prevention of aberrant cell cycle re-entry, which is a hallmark of cellular senescence. By ensuring timely ubiquitin-mediated degradation of mitotic cyclins, APC5 supports genomic stability and prevents the accumulation of cells arrested in aberrant cell cycle states. In yeast, defects in APC/C function lead to chromosome missegregation and genomic instability, both of which shorten replicative lifespan. Additionally, APC/C-mediated proteostasis of cell cycle regulators intersects with nutrient-sensing pathways that modulate chronological and replicative aging in S. cerevisiae.",
"confidence": 0.65,
"key_pathways": [
"APC/C ubiquitin-mediated proteolysis",
"cell cycle regulation and mitotic exit",
"replicative lifespan maintenance"
],
"mechanism_class": "cellular_senescence",
"longevity_influence": "pro_longevity"
}advisor
{
"notes": "The ground truth is \"unclear\" but the model predicted \"pro_longevity\" with 0.65 confidence. The model constructed a plausible-sounding mechanistic argument about APC/C function in cell cycle regulation and genomic stability, but this reasoning is speculative and not supported by direct experimental evidence in yeast aging studies. The model's confidence (0.65) is too high given the actual ambiguity of APC5's role in longevity. The mechanistic reasoning, while biologically coherent in general terms, is not specifically validated for APC5's role in yeast aging, making it hallucinated specifics applied to an unclear case.",
"failure_mode": "confident_wrong",
"answer_correct": false,
"mechanism_correct": false,
"reasoning_quality": 2,
"ground_truth_questionable": false
}