APC9
S. cerevisiae
anaphase promoting complex subunit 9
What the model saw
Gene: APC9 Organism: Saccharomyces cerevisiae Known functions: anaphase promoting complex subunit 9. ubiquitin protein ligase activity.
View unredacted entry
anaphase promoting complex subunit 9. ubiquitin protein ligase activity.
Solver output
APC9 is a subunit of the Anaphase Promoting Complex/Cyclosome (APC/C), an E3 ubiquitin ligase that drives mitotic exit by targeting key substrates such as securin (Pds1) and cyclin B (Clb2/5) for proteasomal degradation. Proper APC/C function is essential for accurate chromosome segregation; loss of APC/C activity leads to mitotic arrest, chromosome mis-segregation, and genomic instability, all of which are associated with shortened replicative lifespan in yeast. By ensuring timely and complete mitotic progression, APC9 supports genomic integrity and orderly cell cycle progression, which are prerequisites for sustained replicative longevity. Additionally, APC/C-mediated ubiquitylation contributes to proteostasis by clearing damaged or misfolded cell-cycle regulators. Thus, the normal function of APC9 is expected to be pro-longevity through maintenance of genomic stability and cell cycle fidelity.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model correctly predicted pro_longevity for APC9. The mechanistic reasoning is sound: APC9 as an APC/C subunit with E3 ubiquitin ligase activity is important for proper mitotic exit, chromosome segregation, and genomic stability, all of which support replicative longevity in yeast. The model correctly identifies key substrates (securin/Pds1, cyclin B/Clb2) and the consequences of APC/C loss. The mechanism class of "genomic_instability" is reasonable, though proteostasis could also be highlighted. Confidence of 0.62 is appropriately moderate given the indirect nature of the inference. Overall solid reasoning with appropriate uncertainty.
Raw API responses
solver
{
"reasoning": "APC9 is a subunit of the Anaphase Promoting Complex/Cyclosome (APC/C), an E3 ubiquitin ligase that drives mitotic exit by targeting key substrates such as securin (Pds1) and cyclin B (Clb2/5) for proteasomal degradation. Proper APC/C function is essential for accurate chromosome segregation; loss of APC/C activity leads to mitotic arrest, chromosome mis-segregation, and genomic instability, all of which are associated with shortened replicative lifespan in yeast. By ensuring timely and complete mitotic progression, APC9 supports genomic integrity and orderly cell cycle progression, which are prerequisites for sustained replicative longevity. Additionally, APC/C-mediated ubiquitylation contributes to proteostasis by clearing damaged or misfolded cell-cycle regulators. Thus, the normal function of APC9 is expected to be pro-longevity through maintenance of genomic stability and cell cycle fidelity.",
"confidence": 0.62,
"key_pathways": [
"APC/C-mediated ubiquitin-proteasome degradation",
"mitotic exit and chromosome segregation",
"cell cycle regulation"
],
"mechanism_class": "genomic_instability",
"longevity_influence": "pro_longevity"
}advisor
{
"notes": "The model correctly predicted pro_longevity for APC9. The mechanistic reasoning is sound: APC9 as an APC/C subunit with E3 ubiquitin ligase activity is important for proper mitotic exit, chromosome segregation, and genomic stability, all of which support replicative longevity in yeast. The model correctly identifies key substrates (securin/Pds1, cyclin B/Clb2) and the consequences of APC/C loss. The mechanism class of \"genomic_instability\" is reasonable, though proteostasis could also be highlighted. Confidence of 0.62 is appropriately moderate given the indirect nature of the inference. Overall solid reasoning with appropriate uncertainty.",
"failure_mode": "correct",
"answer_correct": true,
"mechanism_correct": true,
"reasoning_quality": 4,
"ground_truth_questionable": false
}