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rpl-31

C. elegans

Large ribosomal subunit protein eL31

What the model saw

Gene: rpl-31
Organism: Caenorhabditis elegans
Known functions: Large ribosomal subunit protein eL31. structural constituent of ribosome.
View unredacted entry
Large ribosomal subunit protein eL31. structural constituent of ribosome.

Solver output

Predicted influence
Anti-longevity
Confidence
72%
Mechanism
Deregulated nutrient sensing
Reasoning

rpl-31 encodes the large ribosomal subunit protein eL31, a structural component of the 60S ribosomal subunit. Ribosomal proteins are core effectors of the TOR (Target of Rapamycin) nutrient-sensing pathway, which couples nutrient availability to translational capacity. In C. elegans, reduced ribosomal function — through partial loss-of-function of large subunit proteins — is well established to extend lifespan, likely by attenuating global translation rates and mimicking dietary restriction or TOR inhibition. Downregulation of multiple large ribosomal subunit proteins (e.g., rpl-11.1, rpl-25.1) extends worm lifespan, placing rpl-31's normal pro-translational function in opposition to longevity. The mechanism likely involves reduced proteotoxic burden and improved proteostasis as secondary consequences of lower translational output, but the primary driver is nutrient/TOR-coupled ribosome biogenesis and activity.

Key pathways
TOR signaling / ribosome biogenesistranslational regulationdietary restriction mimicry
Longevity influence
Anti-longevity
Lifespan effect
Increase

GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.

Advisor judgment

Answer
Correct
Mechanism
Correct
Reasoning quality
4/5
Failure mode
Correct
Notes

The model correctly predicted anti_longevity for rpl-31. The mechanistic reasoning is sound: ribosomal large subunit proteins like rpl-31 are anti-longevity because their knockdown reduces translation rates, mimicking TOR inhibition/dietary restriction and extending lifespan. The model correctly identifies that the normal function of rpl-31 (promoting translation) opposes longevity, making it anti-longevity. The mechanism class "deregulated_nutrient_sensing" is a reasonable framing, though "translational regulation" might be more precise as the primary mechanism. The reasoning is well-supported by known C. elegans biology. Confidence of 0.72 is appropriate given the general knowledge about ribosomal proteins in aging.

Raw API responses

solver

{
  "reasoning": "rpl-31 encodes the large ribosomal subunit protein eL31, a structural component of the 60S ribosomal subunit. Ribosomal proteins are core effectors of the TOR (Target of Rapamycin) nutrient-sensing pathway, which couples nutrient availability to translational capacity. In C. elegans, reduced ribosomal function — through partial loss-of-function of large subunit proteins — is well established to extend lifespan, likely by attenuating global translation rates and mimicking dietary restriction or TOR inhibition. Downregulation of multiple large ribosomal subunit proteins (e.g., rpl-11.1, rpl-25.1) extends worm lifespan, placing rpl-31's normal pro-translational function in opposition to longevity. The mechanism likely involves reduced proteotoxic burden and improved proteostasis as secondary consequences of lower translational output, but the primary driver is nutrient/TOR-coupled ribosome biogenesis and activity.",
  "confidence": 0.72,
  "key_pathways": [
    "TOR signaling / ribosome biogenesis",
    "translational regulation",
    "dietary restriction mimicry"
  ],
  "mechanism_class": "deregulated_nutrient_sensing",
  "longevity_influence": "anti_longevity"
}

advisor

{
  "notes": "The model correctly predicted anti_longevity for rpl-31. The mechanistic reasoning is sound: ribosomal large subunit proteins like rpl-31 are anti-longevity because their knockdown reduces translation rates, mimicking TOR inhibition/dietary restriction and extending lifespan. The model correctly identifies that the normal function of rpl-31 (promoting translation) opposes longevity, making it anti-longevity. The mechanism class \"deregulated_nutrient_sensing\" is a reasonable framing, though \"translational regulation\" might be more precise as the primary mechanism. The reasoning is well-supported by known C. elegans biology. Confidence of 0.72 is appropriate given the general knowledge about ribosomal proteins in aging.",
  "failure_mode": "correct",
  "answer_correct": true,
  "mechanism_correct": true,
  "reasoning_quality": 4,
  "ground_truth_questionable": false
}
rpl-31 · Caenorhabditis elegans | Aging biology eval