pat-10
C. elegans
EF-hand domain-containing protein
What the model saw
Gene: pat-10 Organism: Caenorhabditis elegans Known functions: EF-hand domain-containing protein. calcium ion binding. structural constituent of muscle. troponin I binding.
View unredacted entry
EF-hand domain-containing protein. calcium ion binding. structural constituent of muscle. troponin I binding.
Solver output
pat-10 encodes a troponin C-like EF-hand calcium-binding protein that serves as a structural constituent of muscle and binds troponin I, placing it at the core of the thin filament regulatory complex in striated muscle. While muscle integrity and function are important for C. elegans healthspan, troponin C itself acts as a structural/regulatory component of the actomyosin apparatus rather than a direct modulator of canonical longevity pathways (e.g., insulin/IGF-1, TOR, mitochondrial function). Loss of muscle structural integrity can accelerate age-related decline, but overexpression or normal function of a stoichiometric structural protein does not straightforwardly extend lifespan. The calcium-sensing role via EF-hand domains is tied to muscle contraction regulation rather than broader calcium signaling longevity pathways. The net effect on longevity is therefore ambiguous without direct lifespan data.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model predicted "unclear" with low confidence (0.35), but the ground truth is "pro_longevity." The model's reasoning focused on the structural/regulatory role of pat-10 in muscle and argued that such a stoichiometric structural protein wouldn't straightforwardly affect lifespan. This reasoning missed the pro-longevity classification. The model's mechanistic reasoning about muscle integrity and calcium signaling is plausible but incomplete - it failed to consider that muscle structural genes in C. elegans can have direct pro-longevity effects, possibly through maintaining proteostasis, muscle health, or other mechanisms. The model was appropriately uncertain in tone but still got the answer wrong, and the mechanistic reasoning did not capture why pat-10 would be pro-longevity.
Raw API responses
solver
{
"reasoning": "pat-10 encodes a troponin C-like EF-hand calcium-binding protein that serves as a structural constituent of muscle and binds troponin I, placing it at the core of the thin filament regulatory complex in striated muscle. While muscle integrity and function are important for C. elegans healthspan, troponin C itself acts as a structural/regulatory component of the actomyosin apparatus rather than a direct modulator of canonical longevity pathways (e.g., insulin/IGF-1, TOR, mitochondrial function). Loss of muscle structural integrity can accelerate age-related decline, but overexpression or normal function of a stoichiometric structural protein does not straightforwardly extend lifespan. The calcium-sensing role via EF-hand domains is tied to muscle contraction regulation rather than broader calcium signaling longevity pathways. The net effect on longevity is therefore ambiguous without direct lifespan data.",
"confidence": 0.35,
"key_pathways": [
"troponin complex / thin filament regulation",
"calcium ion binding (EF-hand)",
"muscle structural integrity"
],
"mechanism_class": "other",
"longevity_influence": "unclear"
}advisor
{
"notes": "The model predicted \"unclear\" with low confidence (0.35), but the ground truth is \"pro_longevity.\" The model's reasoning focused on the structural/regulatory role of pat-10 in muscle and argued that such a stoichiometric structural protein wouldn't straightforwardly affect lifespan. This reasoning missed the pro-longevity classification. The model's mechanistic reasoning about muscle integrity and calcium signaling is plausible but incomplete - it failed to consider that muscle structural genes in C. elegans can have direct pro-longevity effects, possibly through maintaining proteostasis, muscle health, or other mechanisms. The model was appropriately uncertain in tone but still got the answer wrong, and the mechanistic reasoning did not capture why pat-10 would be pro-longevity.",
"failure_mode": "confident_wrong",
"answer_correct": false,
"mechanism_correct": false,
"reasoning_quality": 2,
"ground_truth_questionable": false
}