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eat-6

C. elegans

Sodium/potassium-transporting ATPase subunit alpha

What the model saw

Gene: eat-6
Organism: Caenorhabditis elegans
Known functions: Sodium/potassium-transporting ATPase subunit alpha. ATP binding. ATP hydrolysis activity. P-type potassium transmembrane transporter activity. P-type sodium:potassium-exchanging transporter activity. nucleotide binding. sodium ion transmembrane transporter activity.
View unredacted entry
Sodium/potassium-transporting ATPase subunit alpha. ATP binding. ATP hydrolysis activity. P-type potassium transmembrane transporter activity. P-type sodium:potassium-exchanging transporter activity. nucleotide binding. sodium ion transmembrane transporter activity.

Solver output

Predicted influence
Pro-longevity
Confidence
65%
Mechanism
Mitochondrial dysfunction
Reasoning

eat-6 encodes the alpha subunit of the Na+/K+-ATPase, the primary ion pump responsible for maintaining electrochemical gradients across the plasma membrane. This pump consumes a large fraction of cellular ATP and is essential for neuronal excitability, nutrient uptake (e.g., amino acid co-transport), and cellular volume regulation. In C. elegans, eat-6 is critical for pharyngeal muscle function and feeding behavior; loss-of-function mutations cause reduced food intake, which mimics dietary restriction—a well-established pro-longevity intervention. Reduced Na+/K+-ATPase activity in eat-6 mutants leads to slowed pumping and caloric restriction-like states that activate nutrient-sensing pathways (e.g., reduced IIS/TOR signaling), extending lifespan. The normal function of eat-6 thus supports robust feeding and energy expenditure, and its partial reduction is associated with longevity through deregulated nutrient sensing rather than complete loss of ion homeostasis.

Key pathways
dietary restriction / caloric restriction mimicryderegulated nutrient sensing (IIS/TOR)Na+/K+-ATPase-dependent ion homeostasis
Longevity influence
Anti-longevity
Lifespan effect
Increase

GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.

Advisor judgment

Answer
Incorrect
Mechanism
Incorrect
Reasoning quality
2/5
Failure mode
Confident wrong
Notes

The model predicted pro_longevity but the ground truth is anti_longevity. The model's reasoning is internally coherent but leads to the wrong conclusion. eat-6 is classified as anti_longevity, meaning its normal function promotes longevity (or its loss shortens lifespan), not that its reduction extends life. The model's logic that eat-6 loss mimics dietary restriction and extends lifespan is incorrect - the gene is anti_longevity, meaning it works against longevity (i.e., its normal activity shortens lifespan, or loss-of-function extends life... wait, actually anti_longevity in GenAge means the gene's normal function promotes aging/shortens lifespan). Actually, in GenAge terminology, anti_longevity means the gene promotes aging (its activity is detrimental to longevity). So the model's reasoning that eat-6 reduction extends lifespan could be consistent with anti_longevity classification - but the model labeled it pro_longevity, which is the opposite of what GenAge says. The model confused the direction: if eat-6 loss extends lifespan, eat-6 would be anti_longevity (its normal function shortens life). The model's mechanistic story (reduced eat-6 → dietary restriction mimicry → longer life) would actually support anti_longevity classification, but the model incorrectly labeled it pro_longevity. Additionally, the mechanism_class of "mitochondrial_dysfunction" is incorrect for a Na+/K+-ATPase pump - this is an ion homeostasis/membrane transport mechanism, not mitochondrial dysfunction. The confidence of 0.65 is moderate but still wrong.

Raw API responses

solver

{
  "reasoning": "eat-6 encodes the alpha subunit of the Na+/K+-ATPase, the primary ion pump responsible for maintaining electrochemical gradients across the plasma membrane. This pump consumes a large fraction of cellular ATP and is essential for neuronal excitability, nutrient uptake (e.g., amino acid co-transport), and cellular volume regulation. In C. elegans, eat-6 is critical for pharyngeal muscle function and feeding behavior; loss-of-function mutations cause reduced food intake, which mimics dietary restriction—a well-established pro-longevity intervention. Reduced Na+/K+-ATPase activity in eat-6 mutants leads to slowed pumping and caloric restriction-like states that activate nutrient-sensing pathways (e.g., reduced IIS/TOR signaling), extending lifespan. The normal function of eat-6 thus supports robust feeding and energy expenditure, and its partial reduction is associated with longevity through deregulated nutrient sensing rather than complete loss of ion homeostasis.",
  "confidence": 0.65,
  "key_pathways": [
    "dietary restriction / caloric restriction mimicry",
    "deregulated nutrient sensing (IIS/TOR)",
    "Na+/K+-ATPase-dependent ion homeostasis"
  ],
  "mechanism_class": "mitochondrial_dysfunction",
  "longevity_influence": "pro_longevity"
}

advisor

{
  "notes": "The model predicted pro_longevity but the ground truth is anti_longevity. The model's reasoning is internally coherent but leads to the wrong conclusion. eat-6 is classified as anti_longevity, meaning its normal function promotes longevity (or its loss shortens lifespan), not that its reduction extends life. The model's logic that eat-6 loss mimics dietary restriction and extends lifespan is incorrect - the gene is anti_longevity, meaning it works against longevity (i.e., its normal activity shortens lifespan, or loss-of-function extends life... wait, actually anti_longevity in GenAge means the gene's normal function promotes aging/shortens lifespan). Actually, in GenAge terminology, anti_longevity means the gene promotes aging (its activity is detrimental to longevity). So the model's reasoning that eat-6 reduction extends lifespan could be consistent with anti_longevity classification - but the model labeled it pro_longevity, which is the opposite of what GenAge says. The model confused the direction: if eat-6 loss extends lifespan, eat-6 would be anti_longevity (its normal function shortens life). The model's mechanistic story (reduced eat-6 → dietary restriction mimicry → longer life) would actually support anti_longevity classification, but the model incorrectly labeled it pro_longevity. Additionally, the mechanism_class of \"mitochondrial_dysfunction\" is incorrect for a Na+/K+-ATPase pump - this is an ion homeostasis/membrane transport mechanism, not mitochondrial dysfunction. The confidence of 0.65 is moderate but still wrong.",
  "failure_mode": "confident_wrong",
  "answer_correct": false,
  "mechanism_correct": false,
  "reasoning_quality": 2
}