RPL21B
S. cerevisiae
60S ribosomal protein eL21 RPL21B
What the model saw
Gene: RPL21B Organism: Saccharomyces cerevisiae Known functions: 60S ribosomal protein eL21 RPL21B. structural constituent of ribosome.
View unredacted entry
60S ribosomal protein eL21 RPL21B. structural constituent of ribosome.
Solver output
RPL21B encodes a structural component of the 60S large ribosomal subunit (eL21), directly contributing to ribosome biogenesis and translational capacity. In yeast, ribosome biogenesis is a major downstream effector of the TOR (Target of Rapamycin) nutrient-sensing pathway; elevated ribosomal activity is associated with shortened lifespan. Deletion of large ribosomal subunit proteins, including several RPL genes, has been shown to extend chronological and replicative lifespan in S. cerevisiae, likely by reducing global translation rates and mimicking caloric restriction or TOR inhibition. Reduced translational output through ribosome limitation also alleviates proteotoxic stress and promotes stress resistance. Therefore, the normal function of RPL21B — supporting full ribosomal assembly and translation — opposes longevity by sustaining high TOR-driven anabolic activity.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model correctly predicted anti_longevity for RPL21B. The mechanistic reasoning is sound and well-articulated: RPL21B encodes a 60S ribosomal subunit protein, and deletion of RPL genes in yeast is known to extend lifespan by reducing translational output, mimicking TOR inhibition/caloric restriction. The mechanism class of "deregulated_nutrient_sensing" is appropriate given the TOR pathway connection. The confidence level of 0.72 is reasonable. Overall, this is an excellent prediction with correct answer and correct mechanistic reasoning.
Raw API responses
solver
{
"reasoning": "RPL21B encodes a structural component of the 60S large ribosomal subunit (eL21), directly contributing to ribosome biogenesis and translational capacity. In yeast, ribosome biogenesis is a major downstream effector of the TOR (Target of Rapamycin) nutrient-sensing pathway; elevated ribosomal activity is associated with shortened lifespan. Deletion of large ribosomal subunit proteins, including several RPL genes, has been shown to extend chronological and replicative lifespan in S. cerevisiae, likely by reducing global translation rates and mimicking caloric restriction or TOR inhibition. Reduced translational output through ribosome limitation also alleviates proteotoxic stress and promotes stress resistance. Therefore, the normal function of RPL21B — supporting full ribosomal assembly and translation — opposes longevity by sustaining high TOR-driven anabolic activity.",
"confidence": 0.72,
"key_pathways": [
"TOR signaling / ribosome biogenesis",
"translational regulation",
"caloric restriction mimicry"
],
"mechanism_class": "deregulated_nutrient_sensing",
"longevity_influence": "anti_longevity"
}advisor
{
"notes": "The model correctly predicted anti_longevity for RPL21B. The mechanistic reasoning is sound and well-articulated: RPL21B encodes a 60S ribosomal subunit protein, and deletion of RPL genes in yeast is known to extend lifespan by reducing translational output, mimicking TOR inhibition/caloric restriction. The mechanism class of \"deregulated_nutrient_sensing\" is appropriate given the TOR pathway connection. The confidence level of 0.72 is reasonable. Overall, this is an excellent prediction with correct answer and correct mechanistic reasoning.",
"failure_mode": "correct",
"answer_correct": true,
"mechanism_correct": true,
"reasoning_quality": 5,
"ground_truth_questionable": false
}