RPL20B
S. cerevisiae
60S ribosomal protein eL20 RPL20B
What the model saw
Gene: RPL20B Organism: Saccharomyces cerevisiae Known functions: 60S ribosomal protein eL20 RPL20B. structural constituent of ribosome.
View unredacted entry
60S ribosomal protein eL20 RPL20B. structural constituent of ribosome.
Solver output
RPL20B encodes a structural component of the 60S ribosomal large subunit (eL20), directly contributing to ribosome biogenesis and translational capacity. In yeast, reduced ribosomal protein gene dosage — particularly of large subunit components — is well-established to extend both replicative and chronological lifespan, largely through attenuation of TORC1-driven protein synthesis. Decreased ribosome abundance mimics nutrient limitation signals, dampening TOR/S6K signaling and promoting longevity-associated responses such as autophagy induction and stress resistance. Deletion of RPL20B specifically has been reported among the longevity-extending interventions in yeast screens, consistent with the broader pattern that reducing 60S ribosomal protein levels extends lifespan. Thus, the normal (wild-type) function of RPL20B — supporting full ribosomal assembly and high translational output — opposes longevity by sustaining elevated TOR signaling and anabolic activity.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model correctly predicted anti_longevity for RPL20B. The mechanistic reasoning is sound: RPL20B encodes a 60S ribosomal protein, and deletion of ribosomal protein genes in yeast is well-established to extend lifespan by reducing translational output and attenuating TOR signaling. The model correctly identifies that the wild-type function of RPL20B opposes longevity. The mechanism class "deregulated_nutrient_sensing" is a reasonable framing, though "translational regulation" or "ribosome biogenesis" might be more precise primary classifications. The reasoning is well-articulated and consistent with the literature on ribosomal protein deletions and yeast longevity. Confidence of 0.72 is appropriate given the well-established pattern for 60S ribosomal proteins.
Raw API responses
solver
{
"reasoning": "RPL20B encodes a structural component of the 60S ribosomal large subunit (eL20), directly contributing to ribosome biogenesis and translational capacity. In yeast, reduced ribosomal protein gene dosage — particularly of large subunit components — is well-established to extend both replicative and chronological lifespan, largely through attenuation of TORC1-driven protein synthesis. Decreased ribosome abundance mimics nutrient limitation signals, dampening TOR/S6K signaling and promoting longevity-associated responses such as autophagy induction and stress resistance. Deletion of RPL20B specifically has been reported among the longevity-extending interventions in yeast screens, consistent with the broader pattern that reducing 60S ribosomal protein levels extends lifespan. Thus, the normal (wild-type) function of RPL20B — supporting full ribosomal assembly and high translational output — opposes longevity by sustaining elevated TOR signaling and anabolic activity.",
"confidence": 0.72,
"key_pathways": [
"TORC1/ribosome biogenesis axis",
"60S large ribosomal subunit assembly",
"translational regulation and nutrient sensing"
],
"mechanism_class": "deregulated_nutrient_sensing",
"longevity_influence": "anti_longevity"
}advisor
{
"notes": "The model correctly predicted anti_longevity for RPL20B. The mechanistic reasoning is sound: RPL20B encodes a 60S ribosomal protein, and deletion of ribosomal protein genes in yeast is well-established to extend lifespan by reducing translational output and attenuating TOR signaling. The model correctly identifies that the wild-type function of RPL20B opposes longevity. The mechanism class \"deregulated_nutrient_sensing\" is a reasonable framing, though \"translational regulation\" or \"ribosome biogenesis\" might be more precise primary classifications. The reasoning is well-articulated and consistent with the literature on ribosomal protein deletions and yeast longevity. Confidence of 0.72 is appropriate given the well-established pattern for 60S ribosomal proteins.",
"failure_mode": "correct",
"answer_correct": true,
"mechanism_correct": true,
"reasoning_quality": 4,
"ground_truth_questionable": false
}