BMH2
S. cerevisiae
14-3-3 family protein BMH2
What the model saw
Gene: BMH2 Organism: Saccharomyces cerevisiae Known functions: 14-3-3 family protein BMH2. DNA replication origin binding. phosphoserine residue binding. protein binding.
View unredacted entry
14-3-3 family protein BMH2. DNA replication origin binding. phosphoserine residue binding. protein binding.
Solver output
BMH2 encodes a yeast 14-3-3 family protein, which are conserved phosphoserine/phosphothreonine-binding scaffold proteins that regulate multiple signaling pathways. In yeast, 14-3-3 proteins (Bmh1/Bmh2) are key regulators of the TORC1 and Ras/PKA nutrient-sensing pathways by binding phosphorylated client proteins and modulating their activity or localization. Specifically, Bmh2 interacts with phosphorylated forms of regulators such as Sch9 and components of the Ras/cAMP/PKA axis, helping to restrain pro-aging nutrient-sensing signals. Loss of BMH2 function has been associated with shortened chronological lifespan in yeast, consistent with a pro-longevity role. Additionally, its DNA replication origin binding activity may contribute to genomic stability, further supporting survival. The 14-3-3 family is broadly conserved as a longevity-associated protein class across eukaryotes.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model correctly predicted pro_longevity for BMH2. The mechanistic reasoning is solid - 14-3-3 proteins in yeast are indeed involved in nutrient-sensing pathways (TORC1, Ras/PKA), and BMH2 loss has been associated with shortened lifespan. The model correctly identifies the phosphoserine-binding scaffold role and its connection to longevity regulation. The confidence of 0.65 is reasonable given the complexity. The mechanism class of "deregulated_nutrient_sensing" is plausible though the actual mechanism may be more nuanced. Overall a well-reasoned correct prediction.
Raw API responses
solver
{
"reasoning": "BMH2 encodes a yeast 14-3-3 family protein, which are conserved phosphoserine/phosphothreonine-binding scaffold proteins that regulate multiple signaling pathways. In yeast, 14-3-3 proteins (Bmh1/Bmh2) are key regulators of the TORC1 and Ras/PKA nutrient-sensing pathways by binding phosphorylated client proteins and modulating their activity or localization. Specifically, Bmh2 interacts with phosphorylated forms of regulators such as Sch9 and components of the Ras/cAMP/PKA axis, helping to restrain pro-aging nutrient-sensing signals. Loss of BMH2 function has been associated with shortened chronological lifespan in yeast, consistent with a pro-longevity role. Additionally, its DNA replication origin binding activity may contribute to genomic stability, further supporting survival. The 14-3-3 family is broadly conserved as a longevity-associated protein class across eukaryotes.",
"confidence": 0.65,
"key_pathways": [
"TORC1/nutrient-sensing signaling",
"Ras/cAMP/PKA pathway",
"DNA replication origin regulation"
],
"mechanism_class": "deregulated_nutrient_sensing",
"longevity_influence": "pro_longevity"
}advisor
{
"notes": "The model correctly predicted pro_longevity for BMH2. The mechanistic reasoning is solid - 14-3-3 proteins in yeast are indeed involved in nutrient-sensing pathways (TORC1, Ras/PKA), and BMH2 loss has been associated with shortened lifespan. The model correctly identifies the phosphoserine-binding scaffold role and its connection to longevity regulation. The confidence of 0.65 is reasonable given the complexity. The mechanism class of \"deregulated_nutrient_sensing\" is plausible though the actual mechanism may be more nuanced. Overall a well-reasoned correct prediction.",
"failure_mode": "correct",
"answer_correct": true,
"mechanism_correct": true,
"reasoning_quality": 4,
"ground_truth_questionable": false
}