CKA2
S. cerevisiae
casein kinase 2 catalytic subunit CKA2
What the model saw
Gene: CKA2 Organism: Saccharomyces cerevisiae Known functions: casein kinase 2 catalytic subunit CKA2. ATP binding. protein binding. protein kinase activity. protein serine kinase activity. protein serine/threonine kinase activity.
View unredacted entry
casein kinase 2 catalytic subunit CKA2. ATP binding. protein binding. protein kinase activity. protein serine kinase activity. protein serine/threonine kinase activity.
Solver output
CKA2 encodes a catalytic subunit of Casein Kinase 2 (CK2), a constitutively active Ser/Thr kinase that phosphorylates numerous substrates involved in cell growth and proliferation. In yeast, CK2 activity promotes ribosome biogenesis and protein synthesis downstream of nutrient-sensing pathways, functionally overlapping with the TOR/PKA growth-signaling axis. Elevated CK2 activity sustains anabolic signaling and suppresses stress-response and autophagy programs that are pro-longevity. Genetic studies in yeast have shown that reducing CK2 activity extends chronological and/or replicative lifespan, consistent with its role as a growth-promoting kinase that antagonizes longevity. Thus, the normal (active) function of CKA2 opposes longevity by maintaining high nutrient-sensing/growth signaling output.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model correctly predicted CKA2 as anti_longevity. The mechanistic reasoning is sound and well-grounded: CK2 is a constitutively active kinase that promotes growth signaling, ribosome biogenesis, and suppresses stress responses, all of which are consistent with an anti-longevity role. The model correctly connects CK2 to the TOR/PKA nutrient-sensing axis and notes that reducing CK2 activity extends lifespan in yeast. The confidence level of 0.72 is appropriate. The mechanism class "deregulated_nutrient_sensing" is a reasonable categorization, though CK2 is perhaps more directly a growth-promoting kinase than a nutrient sensor per se. Overall, the reasoning is coherent and well-supported.
Raw API responses
solver
{
"reasoning": "CKA2 encodes a catalytic subunit of Casein Kinase 2 (CK2), a constitutively active Ser/Thr kinase that phosphorylates numerous substrates involved in cell growth and proliferation. In yeast, CK2 activity promotes ribosome biogenesis and protein synthesis downstream of nutrient-sensing pathways, functionally overlapping with the TOR/PKA growth-signaling axis. Elevated CK2 activity sustains anabolic signaling and suppresses stress-response and autophagy programs that are pro-longevity. Genetic studies in yeast have shown that reducing CK2 activity extends chronological and/or replicative lifespan, consistent with its role as a growth-promoting kinase that antagonizes longevity. Thus, the normal (active) function of CKA2 opposes longevity by maintaining high nutrient-sensing/growth signaling output.",
"confidence": 0.72,
"key_pathways": [
"TOR/CK2 nutrient-sensing and ribosome biogenesis",
"protein serine/threonine phosphorylation promoting cell growth",
"stress-response suppression via CK2-mediated phosphorylation"
],
"mechanism_class": "deregulated_nutrient_sensing",
"longevity_influence": "anti_longevity"
}advisor
{
"notes": "The model correctly predicted CKA2 as anti_longevity. The mechanistic reasoning is sound and well-grounded: CK2 is a constitutively active kinase that promotes growth signaling, ribosome biogenesis, and suppresses stress responses, all of which are consistent with an anti-longevity role. The model correctly connects CK2 to the TOR/PKA nutrient-sensing axis and notes that reducing CK2 activity extends lifespan in yeast. The confidence level of 0.72 is appropriate. The mechanism class \"deregulated_nutrient_sensing\" is a reasonable categorization, though CK2 is perhaps more directly a growth-promoting kinase than a nutrient sensor per se. Overall, the reasoning is coherent and well-supported.",
"failure_mode": "correct",
"answer_correct": true,
"mechanism_correct": true,
"reasoning_quality": 4,
"ground_truth_questionable": false
}