RPL12B
S. cerevisiae
60S ribosomal protein uL11 RPL12B
What the model saw
Gene: RPL12B Organism: Saccharomyces cerevisiae Known functions: 60S ribosomal protein uL11 RPL12B. large ribosomal subunit rRNA binding. structural constituent of ribosome.
View unredacted entry
60S ribosomal protein uL11 RPL12B. large ribosomal subunit rRNA binding. structural constituent of ribosome.
Solver output
RPL12B encodes a structural component of the 60S large ribosomal subunit (uL11), directly involved in rRNA binding and ribosome assembly. Ribosomal protein genes in yeast are tightly coupled to TOR (Target of Rapamycin) signaling, the master nutrient-sensing pathway; high TOR activity upregulates ribosome biogenesis and translation, which is associated with reduced lifespan. Deletion of many large ribosomal subunit proteins, including RPL components, has been shown to extend chronological and replicative lifespan in S. cerevisiae, likely by mimicking reduced TOR signaling and decreasing global translation rates. Reduced translational capacity through ribosome limitation can also activate stress-response pathways and improve proteostasis. Thus, the normal pro-growth function of RPL12B — promoting ribosome assembly and translation — opposes longevity by sustaining high TOR-driven anabolic activity.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model correctly predicted anti_longevity for RPL12B. The mechanistic reasoning is sound and well-articulated: RPL12B encodes a 60S ribosomal subunit protein, and deletion of ribosomal protein genes in yeast is well-established to extend lifespan, likely through reduced TOR signaling and decreased global translation rates. The model correctly identifies that the normal function of RPL12B (promoting ribosome assembly and translation) opposes longevity, making it anti-longevity. The mechanism class of "deregulated_nutrient_sensing" is reasonable given the TOR connection, though "translational regulation" might be more directly accurate. The confidence level of 0.72 is appropriate given the general knowledge about RPL genes in yeast aging.
Raw API responses
solver
{
"reasoning": "RPL12B encodes a structural component of the 60S large ribosomal subunit (uL11), directly involved in rRNA binding and ribosome assembly. Ribosomal protein genes in yeast are tightly coupled to TOR (Target of Rapamycin) signaling, the master nutrient-sensing pathway; high TOR activity upregulates ribosome biogenesis and translation, which is associated with reduced lifespan. Deletion of many large ribosomal subunit proteins, including RPL components, has been shown to extend chronological and replicative lifespan in S. cerevisiae, likely by mimicking reduced TOR signaling and decreasing global translation rates. Reduced translational capacity through ribosome limitation can also activate stress-response pathways and improve proteostasis. Thus, the normal pro-growth function of RPL12B — promoting ribosome assembly and translation — opposes longevity by sustaining high TOR-driven anabolic activity.",
"confidence": 0.72,
"key_pathways": [
"TOR signaling / ribosome biogenesis",
"translational regulation",
"60S ribosomal subunit assembly"
],
"mechanism_class": "deregulated_nutrient_sensing",
"longevity_influence": "anti_longevity"
}advisor
{
"notes": "The model correctly predicted anti_longevity for RPL12B. The mechanistic reasoning is sound and well-articulated: RPL12B encodes a 60S ribosomal subunit protein, and deletion of ribosomal protein genes in yeast is well-established to extend lifespan, likely through reduced TOR signaling and decreased global translation rates. The model correctly identifies that the normal function of RPL12B (promoting ribosome assembly and translation) opposes longevity, making it anti-longevity. The mechanism class of \"deregulated_nutrient_sensing\" is reasonable given the TOR connection, though \"translational regulation\" might be more directly accurate. The confidence level of 0.72 is appropriate given the general knowledge about RPL genes in yeast aging.",
"failure_mode": "correct",
"answer_correct": true,
"mechanism_correct": true,
"reasoning_quality": 5,
"ground_truth_questionable": false
}