BPT1
S. cerevisiae
ATP-binding cassette bilirubin transporter BPT1
What the model saw
Gene: BPT1 Organism: Saccharomyces cerevisiae Known functions: ATP-binding cassette bilirubin transporter BPT1. ABC-type transporter activity. ATP binding. ATP hydrolysis activity. cadmium ion transmembrane transporter activity. transmembrane transporter activity.
View unredacted entry
ATP-binding cassette bilirubin transporter BPT1. ABC-type transporter activity. ATP binding. ATP hydrolysis activity. cadmium ion transmembrane transporter activity. transmembrane transporter activity.
Solver output
BPT1 encodes a vacuolar ABC transporter in S. cerevisiae that mediates ATP-dependent sequestration of toxic compounds—including cadmium ions and glutathione-conjugated substrates (analogous to bilirubin/organic anion conjugates)—into the vacuole. By actively removing heavy metal ions (e.g., Cd²⁺) and reactive metabolic byproducts from the cytoplasm, BPT1 reduces oxidative and proteotoxic stress that would otherwise accelerate cellular aging. This detoxification function is mechanistically linked to glutathione conjugate export, a key arm of the cellular stress response. Loss of such vacuolar sequestration capacity in yeast is associated with increased sensitivity to metal-induced damage and shortened replicative lifespan under stress conditions. Therefore, BPT1's normal function is expected to be pro-longevity by limiting intracellular accumulation of cytotoxic species.
GenAge curators classify each gene's normal function as promoting (Pro-Longevity) or opposing (Anti-Longevity) longevity, based on synthesis across studies. Loss-of-function effects can vary from this depending on the manipulation.
Advisor judgment
The model predicted pro_longevity but the ground truth is anti_longevity. The model's reasoning is plausible on the surface (detoxification transporters could extend lifespan), but it is incorrect. BPT1 is actually anti-longevity in yeast, meaning its deletion or loss of function extends lifespan rather than shortening it. The model constructed a logical-sounding but ultimately wrong mechanistic argument, claiming that BPT1's detoxification role would protect against aging. The model had moderate confidence (0.62) but was still wrong. The reasoning about vacuolar sequestration of toxic compounds being beneficial is not supported by the actual experimental data for this gene.
Raw API responses
solver
{
"reasoning": "BPT1 encodes a vacuolar ABC transporter in S. cerevisiae that mediates ATP-dependent sequestration of toxic compounds—including cadmium ions and glutathione-conjugated substrates (analogous to bilirubin/organic anion conjugates)—into the vacuole. By actively removing heavy metal ions (e.g., Cd²⁺) and reactive metabolic byproducts from the cytoplasm, BPT1 reduces oxidative and proteotoxic stress that would otherwise accelerate cellular aging. This detoxification function is mechanistically linked to glutathione conjugate export, a key arm of the cellular stress response. Loss of such vacuolar sequestration capacity in yeast is associated with increased sensitivity to metal-induced damage and shortened replicative lifespan under stress conditions. Therefore, BPT1's normal function is expected to be pro-longevity by limiting intracellular accumulation of cytotoxic species.",
"confidence": 0.62,
"key_pathways": [
"vacuolar detoxification / glutathione-conjugate sequestration",
"cadmium ion transmembrane transport",
"ABC transporter-mediated xenobiotic clearance"
],
"mechanism_class": "other",
"longevity_influence": "pro_longevity"
}advisor
{
"notes": "The model predicted pro_longevity but the ground truth is anti_longevity. The model's reasoning is plausible on the surface (detoxification transporters could extend lifespan), but it is incorrect. BPT1 is actually anti-longevity in yeast, meaning its deletion or loss of function extends lifespan rather than shortening it. The model constructed a logical-sounding but ultimately wrong mechanistic argument, claiming that BPT1's detoxification role would protect against aging. The model had moderate confidence (0.62) but was still wrong. The reasoning about vacuolar sequestration of toxic compounds being beneficial is not supported by the actual experimental data for this gene.",
"failure_mode": "confident_wrong",
"answer_correct": false,
"mechanism_correct": false,
"reasoning_quality": 2,
"ground_truth_questionable": false
}